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open reading frame encoding icre  (TaKaRa)


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    Structured Review

    TaKaRa open reading frame encoding icre
    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted <t>open</t> <t>reading</t> <t>frame</t> (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Open Reading Frame Encoding Icre, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/open+reading+frame+encoding+icre/BamH+I/pmc12800110-193-1-30
    Average 99 stars, based on 14704 article reviews
    open reading frame encoding icre - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia"

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-31077-z

    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Figure Legend Snippet: Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Techniques Used: Injection, Expressing

    Related Articles

    Amplification:

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Polymerase Chain Reaction:

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Ligation:

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Cloning:

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia
    Article Snippet: Animals were bred with WT LE rats from Charles River Laboratories. .. The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech). .. The resulting plasmid (pOTTC556, Addgene; #210416) was grown in Stbl3 cells (Invitrogen) and verified by sequencing before packaging into AAV particles.



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    open reading frame encoding icre  (TaKaRa)
    99
    TaKaRa open reading frame encoding icre
    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted <t>open</t> <t>reading</t> <t>frame</t> (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Open Reading Frame Encoding Icre, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/open+reading+frame+encoding+icre/BamH+I/pmc12800110-193-1-30
    Average 99 stars, based on 1 article reviews
    open reading frame encoding icre - by Bioz Stars, 2026-10
    99/100 stars
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    open reading frame encoding icre  (Addgene inc)
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    Addgene inc open reading frame encoding icre
    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted <t>open</t> <t>reading</t> <t>frame</t> (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .
    Open Reading Frame Encoding Icre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/open+reading+frame+encoding+icre/pOTTC293+-+pAAV+EF1a+V5-synuclein+(WT)+(Plasmid+%2360057)/pmc12800110-193-1-24
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    Image Search Results


    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Journal: Scientific Reports

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1038/s41598-025-31077-z

    Figure Lengend Snippet: Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Article Snippet: The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech).

    Techniques: Injection, Expressing

    Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Journal: Scientific Reports

    Article Title: Generation and characterization of a tamoxifen-inducible, Cre driver rat for transgene expression in microglia

    doi: 10.1038/s41598-025-31077-z

    Figure Lengend Snippet: Cx3cr1-CreERT2 rat creation and characterization overview. ( A ) The Cx3cr1-CreERT2 DNA was injected into Long Evans (LE) rat embryos. ( B ) The double-floxed inverted open reading frame (DIO) was used to produce the DIO-mCherry rat for Cre-dependent expression of the mCherry fluorescent protein in any cells that express Cre. ( C ) The DIO-mCherry rat was crossed with the Cx3cr1-CreERT2 and treated with tamoxifen (TAM) to fluorescently label Cx3cr1 expressing cells such as microglia in the brain. Created in BioRender. Harvey, B. (2025) https://BioRender.com/lf2aj0o .

    Article Snippet: The open-reading-frame encoding iCre (based on pBSII-iCre , was amplified by PCR with linkered primers and inserted the BamHI and EcoRI sites of pOTTC293 (Addgene; #60057) using ligation-independent cloning (In-Fusion; Clontech).

    Techniques: Injection, Expressing